Assessment of Transcriptomic and Apical Responses of Daphnia magna Exposed to a Polyethylene Microplastic in a 21‐d Chronic Study. (9th June 2020)

• Record Type: Journal Article
• Authors: Coady, Katherine K.; Burgoon, Lyle; Doskey, Claire; Davis, John W.
• Is Part Of: Environmental toxicology and chemistry. Volume 39:Number 8(2020)
• Page Start: 1578
• Page End: 1589
• Publication Date: 2020-06-09
• Subjects: Daphnia magna -- Microplastic -- Transcriptome -- Aquatic toxicity; Pollution -- Environmental aspects -- Periodicals; Environmental chemistry -- Periodicals; 615.902
• DOI: 10.1002/etc.4745
• Languages: English
• ISSNs: 0730-7268

Abstract

Abstract: There is global concern regarding the fate and effects of microplastics in the environment, particularly in aquatic systems. In the present study, ethylene acrylic acid copolymer particles were evaluated in a chronic toxicity study with the aquatic invertebrate Daphnia magna . The study design included a natural particle control treatment (silica) to differentiate any potential physical effects of a particle from the intrinsic toxicity of the test material. In addition to the standard endpoints of survival, growth, and reproduction, the transcriptomic profiles of control and ethylene acrylic acid copolymer‐exposed D. magna were evaluated at the termination of the 21‐d toxicity study. No significant effects on D. magna growth, survival, or reproduction were observed in comparison with both particle and untreated control groups. Significant transcriptomic alterations were induced at the highest treatment level of 2.3 × 10 12 particles of the ethylene acrylic acid copolymer/L in key pathways linked to central metabolism and energy reserves, oxidative stress, and ovulation and molting, indicating a global transcriptomic response pattern. To put the results in perspective is challenging at this time, because, to date, microplastic environmental monitoring approaches have not been equipped to detect particles in the nanosize range. However, our results indicate that ethylene acrylic acid copolymer microplastics in the upper nanosize range are not expected to adversely affect D. magna growth, survival, or reproductive outcomes at concentrations of up to 10 12 particles/L. Environ Toxicol Chem 2020;39:1578–1589. © 2020 SETAC