MiR-9875 functions in antiviral immunity by targeting PDCD6 in mud crab (Scylla paramamosain). Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Gong, Yi; Kong, Tongtong; Ren, Xin; Lin, Shanmeng; Li, Shengkang
• Is Part Of: Virulence. Volume 11:Issue 1(2020)
• Page Start: 849
• Page End: 862
• Publication Date: 2020-01-01
• Subjects: miR-9875 -- PDCD6 -- apoptosis -- WSSV -- Scylla paramamosain; Virulence (Microbiology) -- Periodicals; Bacterial diseases -- Periodicals; Molecular microbiology -- Periodicals; 579.05
• DOI: 10.1080/21505594.2020.1787078
• Languages: English
• ISSNs: 2150-5608

Abstract

ABSTRACT: Programmed cell death 6 (PDCD6) is a well-known apoptosis regulator that is involved in the immunity of mammals. However, the effects of miRNA-mediated regulation of PDCD6 expression on apoptosis and virus infection in organisms, especially in marine invertebrates, have not been extensively explored. In this study, PDCD6 of mud crab ( Scylla paramamosain ) ( Sp -PDCD6) was characterized. The results showed that Sp -PDCD6 contains five EF-hands domains and could suppress virus infection via apoptosis promotion. It also presented that Sp -PDCD6 was directly targeted by miR-9875 in vitro and in vivo, miR-9875 served as a positive regulator during the virus invasion. The findings indicated that the miR-9875-PDCD6 pathway possessed fundamental effects on the immune response to virus infection in mud crab. Therefore, our research provided a novel insight into the roles of both miR-9875 and PDCD6 in the regulation of apoptosis and virus defense in mud crab.