2-Hydroxymelatonin induced nutritional orchestration in Cucumis sativus under cadmium toxicity: modulation of non-enzymatic antioxidants and gene expression. Issue 5 (15th April 2020)

• Record Type: Journal Article
• Authors: Shah, Anis Ali; Ahmed, Shakil; Yasin, Nasim Ahmad
• Is Part Of: International journal of phytoremediation. Volume 22:Issue 5(2020)
• Page Start: 497
• Page End: 507
• Publication Date: 2020-04-15
• Subjects: Antioxidants -- cadmium -- Cucumis sativus -- 2-hydroxymelatonin -- nutrients; Phytoremediation -- Periodicals; 628.5
• DOI: 10.1080/15226514.2019.1683715
• Languages: English
• ISSNs: 1522-6514

Abstract

Abstract: 2-Hydroxymelatonin (2-OHMT) is an important metabolite produced through melatonin interaction with oxygenated compounds. 2-OHMT pretreated seeds (50 µM, 100 µM, and 150 µM) were grown in soil contaminated with 50 mg kg −1 cadmium. Cadmium imposed stress reduced seed germination, growth, biomass production, and chlorophyll (Chl) content in Cucumis sativus seedlings. 2-OHMT application emphatically revamped germination, shoot length, root length, and plant biomass production. The 2-OHMT pretreatment modulated expression levels of plasma membrane H + -ATPase genes of C. sativus including CsHA2, CsHA3, CsHA4, CsHA8, and CsHA9 . This biomolecule amplified the accumulation of antioxidants such as glutathione, proline, phenolics, and flavonoids. The reduced Cd-uptake in 2-OHMT treated C. sativus seedlings encouraged uptake of essential plant nutrients. Furthermore, conjugated increase of indole acetic acid contents and ethylene production rate were observed in 2-OHMT treated seedlings in a dose-dependent manner. The improved nutritional content in 2-OHMT applied seedlings was ascribed to enhanced expression of H + -ATPase regulating genes besides increased amount of non-enzymatic antioxidants in Cd-stressed plants. The present novel study elucidates the potential of 2-OHMT in improving nutritional content in cucumber plants by modulation of non-enzymatic antioxidants and gene expression.