Identification and characterization of a novel gene-encoded antioxidant peptide obtained from amphibian skin secretions. Issue 6 (18th March 2020)

• Record Type: Journal Article
• Authors: Wang, Ying; Cao, Xiaoqing; Fu, Zhe; Wang, Siyuan; Li, Xiaojie; Liu, Naixin; Feng, Zhuo; Yang, Meifeng; Tang, Jing; Yang, Xinwang
• Is Part Of: Natural product research. Volume 34:Issue 6(2020)
• Page Start: 754
• Page End: 758
• Publication Date: 2020-03-18
• Subjects: Odorrana margaretae -- OM-GF17 -- antioxidant peptide -- molecular basis; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1499635
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Amphibian skin is known to secrete gene-encoded antioxidant peptides of small molecular weight, which play important roles in host defense. However, recognition of such peptides is still in its infancy. Here, we discovered a novel gene-encoded antioxidant peptide (named OM-GF17) from skin secretions of amphibian species, Odorrana margaretae . Produced by the post-translational processing of a 61-residue prepropeptide, the amino acid sequence of OM-GF17 was 'GFFKWHPRCGEEHSMWT', with a molecular mass of 2135.7 Da. Functional analysis revealed that OM-GF17 scavenged ABTS +, DPPH, NO and decreased iron oxidation. Our results also implied that five amino acid residues, including Cys, Pro, Met, Trp, and Phe, be related to the antioxidant activity of OM-GF17. Furthermore, OM-GF17 did not exhibit direct microbe-killing activity. This novel gene-encoded antioxidant peptide could help in the development of new antioxidant agents and increase our understanding of the biological functions of amphibian skin.