AtPPRT1 negatively regulates salt stress response in Arabidopsis seedlings. (3rd March 2020)

• Record Type: Journal Article
• Authors: Liu, Yu; Pei, Linsen; Xiao, Shuya; Peng, Lu; Liu, Zhibin; Li, Xufeng; Yang, Yi; Wang, Jianmei
• Is Part Of: Plant signaling & behavior. Volume 15:Number 3(2020)
• Publication Date: 2020-03-03
• Subjects: Arabidopsis -- AtPPRT1 -- salt tolerance; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2020.1732103
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: Salt stress is one of the environmental factors that negatively affect plant growth and development. We have previously reported a putative C3HC4 zinc-finger ubiquitin E3 ligase (AtPPRT1) negatively regulates Abscisic acid (ABA) and drought stress response. According to previous studies, the accumulation of ABA in plants can further regulate the salt stress response. Therefore, in this study, we further analyzed whether AtPPRT1 negatively regulates the salt stress response. The results showed that AtPPRT1 expression was induced by salt stress. Furthermore, under salt stress, the β-glucuronidase ( GUS ) gene driven by the AtPPRT1 promoter has shown increased activity in the hypocotyl and petioles of Arabidopsis seedlings. Additionally, seedlings of the T-DNA insertion mutant atpprt1 showed significant growth advantage under salt stress, whereas overexpressing AtPPRT1 (OE lines) in Arabidopsis seedlings displayed hypersensitive under salt stress. Etiolated atpprt1 seedlings also demonstrated significantly elongated hypocotyl lengths in salt stress. The elevated or reduced salt tolerance of atpprt1 and AtPPRT1 overexpressing lines was confirmed by the changes in chlorophyll content and 3, 3ʹ-Diaminobenzidine (DAB) staining. The above data suggest that AtPPRT1 has a negative effect on salt tolerance in Arabidopsis seedlings.