Allergen false-detection using official bioinformatic algorithms. Issue 2 (2nd April 2020)

• Record Type: Journal Article
• Authors: Herman, Rod A.; Song, Ping
• Is Part Of: GM crops and food. Volume 11:Issue 2(2020)
• Page Start: 93
• Page End: 96
• Publication Date: 2020-04-02
• Subjects: Allergen -- cross-reactivity -- bioinformatics -- selectivity -- exposure; Crops -- Genetic engineering -- Periodicals; Agriculture -- Periodicals; Food industry and trade -- Periodicals; 631.5233
• DOI: 10.1080/21645698.2019.1709021
• Languages: English
• ISSNs: 2164-5701

Abstract

ABSTRACT: Bioinformatic amino acid sequence searches are used, in part, to assess the potential allergenic risk of newly expressed proteins in genetically engineered crops. Previous work has demonstrated that the searches required by government regulatory agencies falsely implicate many proteins from rarely allergenic crops as an allergenic risk. However, many proteins are found in crops at concentrations that may be insufficient to cause allergy. Here we used a recently developed set of high-abundance non-allergenic proteins to determine the false-positive rates for several algorithms required by regulatory bodies, and also for an alternative 1:1 FASTA approach previously found to be equally sensitive to the official sliding-window method, but far more selective. The current investigation confirms these earlier findings while addressing dietary exposure.