HHC-36 antimicrobial peptide loading on silk fibroin (SF)/hydroxyapatite (HA) nanofibrous-coated titanium for the enhancement of osteoblast and bactericidal functions. Issue 10 (2nd July 2020)

• Record Type: Journal Article
• Authors: Abbasizadeh, Nastaran; Rezayan, Ali Hossein; Nourmohammadi, Jhamak; Kazemzadeh-Narbat, Mehdi
• Is Part Of: International journal of polymeric materials. Volume 69:Issue 10(2020)
• Page Start: 629
• Page End: 639
• Publication Date: 2020-07-02
• Subjects: Antimicrobial peptide -- hydroxyapatite nanoparticles -- silk fibroin fibers -- titanium implant; Polymers -- Periodicals; Plastics -- Periodicals; 620.19205
• DOI: 10.1080/00914037.2019.1596913
• Languages: English
• ISSNs: 0091-4037

Abstract

Abstract: Improving osseointegration together with preventing infections are the main challenges of using titanium-based implants in joint replacement surgeries. In this study, a hybrid composite of silk fibroin (SF)/hydroxyapatite (HA) nanofibrous mats was directly electrospun on titanium plates in order to mimic the bone extracellular structure and improve osteoconductivity. Afterwards, HHC-36 antimicrobial peptide (AMP) was immobilized on the fabricated mats using dopamine as a linker. The morphology, bioactivity, and adhesive strength of the coating were characterized, and the cellular responses and antimicrobial activity of the modified titanium plates were assessed. The results exhibited a strong interfacial attachment and homogenously dispersed HA nanoparticles in the electrospun nanofibers. No cytotoxicity was observed for MG-63 cells and the coating could effectively induce cell differentiation. The cumulative release profile of HHC-36 showed a burst release within first 3 h, followed by a slow and steady release over 48 h. Finally, AMP could eradicate the representative Gram-positive and Gram-negative bacteria within 3 h while maintaining the antimicrobial activity for up to 21 days. GRAPHICAL ABSTRACT: (A) The SEM morphology and fiber diameter distribution of electrospun SF/HA nanofibers after modification with (a, b, c) PDA and (d, e, f) HHC-36, and (B) Assessing the antibacterial activity of (1) Ti/SF/HA/AMP (2) Ti/SF/HA and (3) Ti against E. Coli and S. aurues by Disk Diffusion test