Antiproliferative effect of 2-Hydroxy-6-tridecylbenzoic acid from ginkgo biloba sarcotestas through the aryl hydrocarbon receptor pathway in triple-negative breast cancer cells. Issue 6 (18th March 2020)

• Record Type: Journal Article
• Authors: Zhou, Dayu; Jiang, Chunying; Fu, Chenghao; Chang, Ping; Yang, Bin; Wu, Jiadi; Zhao, Xiaohui; Ma, Shiliang
• Is Part Of: Natural product research. Volume 34:Issue 6(2020)
• Page Start: 893
• Page End: 897
• Publication Date: 2020-03-18
• Subjects: Breast cancer -- ginkgo biloba sarcotestas -- 2-Hydroxy-6-tridecylbenzoic acid -- antiproliferation -- CYP1B1 -- AhR; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1508144
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: This study aims to isolate the potential antiproliferative and cytotoxic compounds from ginkgo biloba sarcotestas (GBS) and investigates the underlying mechanism in human MDA-MB-231 and mouse 4T-1 triple-negative breast cancer cells. Our results showed that 2-Hydroxy-6-tridecylbenzoic acid was isolated by cytotoxicity-guided fractionation where different fractions were assessed using MTT assay against MDA-MB-231 and 4T-1 cells. Colony formation assay showed that 2-Hydroxy-6-tridecylbenzoic acid significantly inhibited cell proliferation. The inhibition was associated with the enhancement of cytochrome P450 (CYP) 1B1 expression in a dose- and time-dependent manner and no significant change of CYP1A1 expression by qPCR and Western blot assays in MDA-MB-231 and 4T-1 cells. The mechanism was further demonstrated by the activation of aryl hydrocarbon receptor (AhR) pathway with the upregulation of AhR, AhR nuclear translocator (ARNT) and AhR-dependent xenobiotic response elements (XRE) activity. These findings may have implications for development of anticancer agents containing 2-Hydroxy-6-tridecylbenzoic acid as functional additives. Graphical Abstract: