Impaired expression of innate immunity-related genes in IgG4-related disease: A possible mechanism in the pathogenesis of IgG4-RD. Issue 3 (3rd May 2020)

• Record Type: Journal Article
• Authors: Nakamura, Takuji; Satoh-Nakamura, Tomomi; Nakajima, Akio; Kawanami, Takafumi; Sakai, Tomoyuki; Fujita, Yoshimasa; Iwao, Haruka; Miki, Miyuki; Masaki, Yasufumi; Okazaki, Toshiro; Ishigaki, Yasuhito; Kawano, Mitsuhiro; Yamada, Kazunori; Matsui, Shoko; Saeki, Takako; Kamisawa, Terumi; Yamamoto, Motohisa; Hamano, Hideaki; Origuchi, Tomoki; Hirata, Shintaro; Tanaka, Yoshiya; Tsuboi, Hiroto; Sumida, Takayuki; Okazaki, Kazuichi; Tanaka, Masao; Chiba, Tsutomu; Mimori, Tsuneyo; Umehara, Hisanori
• Is Part Of: Modern rheumatology. Volume 30:Issue 3(2020)
• Page Start: 551
• Page End: 557
• Publication Date: 2020-05-03
• Subjects: IgG4-related disease -- DNA microarray analysis -- innate immunity related gene -- innate immunity -- acquired immunity; Rheumatology -- Periodicals; 616.723005
• DOI: 10.1080/14397595.2019.1621475
• Languages: English
• ISSNs: 1439-7595

Abstract

Abstract: Background: IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and tissue infiltration by IgG4-positive plasma cells. The pathogenesis of this disease is not clear. Transcriptome analysis was performed to identify genes over- and under-expressed in patients with IgG4-RD. Method: DNA microarray analysis was performed using RNA from peripheral blood mononuclear cells of two patients with IgG4-RD and four healthy individuals. Genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy were identified. Four genes related to innate immunity such as transcobalamin I (TCN1), secretory leukocyte peptidase inhibitor (SLPI), bactericidal/permeability-increasing protein (BPI) and lactotransferrin (LTF) were assessed by real-time PCR in 15 IgG4-RD patients and 13 healthy individuals. Result: DNA microarray analysis identified 30 genes showing a greater than threefold change in expression in IgG4-RD patients following steroid therapy. Real-time RT-PCR showed that the levels of mRNAs encoding TCNI and SLPI, except for BPI and LTF, were significantly lower in patients with IgG4-RD than in healthy people. The levels of all four mRNAs in patients with IgG4-RD were significantly increased after steroid treatment. Conclusion: These results indicate that reduction in expression of innate immunity-related genes may participate in the pathogenesis of IgG4-RD that steroid treatment may rectify impaired innate immunity as well as acquired immunity.