Interleukin-6 measured using the automated electrochemiluminescence immunoassay method for the identification of intra-amniotic inflammation in preterm prelabor rupture of membranes. (2nd June 2020)

• Record Type: Journal Article
• Authors: Musilova, Ivana; Andrys, Ctirad; Holeckova, Magdalena; Kolarova, Veronika; Pliskova, Lenka; Drahosova, Marcela; Bolehovska, Radka; Pilka, Radovan; Huml, Karel; Cobo, Teresa; Jacobsson, Bo; Kacerovsky, Marian
• Is Part Of: Journal of maternal-fetal & neonatal medicine. Volume 33:Number 11(2020)
• Page Start: 1919
• Page End: 1926
• Publication Date: 2020-06-02
• Subjects: Amniotic fluid -- cytokine -- immunoassay method -- marker -- preterm birth; Obstetrics -- Periodicals; Perinatology -- Periodicals; Infants (Newborn) -- Diseases -- Periodicals; Neonatology -- Periodicals; 618.2
• DOI: 10.1080/14767058.2018.1533947
• Languages: English
• ISSNs: 1476-7058

Abstract

Abstract: Introduction: We aimed to compare the amniotic fluid interleukin (IL)-6 concentrations measured using the automated electrochemiluminescence immunoassay method and ELISA, and to establish an IL-6 concentration cut-off value for intra-amniotic inflammation (IAI) in preterm prelabor rupture of membranes (PPROM), which can be used in the automated electrochemiluminescence immunoassay method. Materials and methods: A total of 120 women with PPROM were included in this study. Amniotic fluid samples were obtained through transabdominal amniocentesis. IL-6 concentrations were assessed using both the automated electrochemiluminescence immunoassay method and ELISA, the current gold standard. IAI was defined as an amniotic fluid IL-6 concentration of ≥2600 pg/mL measured using ELISA. Results: A correlation between both assays was found (Spearman's rho = 0.97; p < .0001). Based on the receiver-operating characteristic curve for the identification of IAI (area under the curve = 0.99), a cut-off value of ≥3000 pg/mL was selected for the automated electrochemiluminescence immunoassay method with a sensitivity of 88%, specificity of 99%, positive predictive value of 97%, negative predictive value of 96%, and likelihood ratio of 76. Conclusions: For amniotic fluid IL-6 concentrations assessed using the automated electrochemiluminescence immunoassay method, a cut-off value of 3000 pg/mL was indicated for diagnosing IAI in women with PPROM.