Effect of temperature on phenotype characterization of Ralstonia solanacearum from tobacco. Issue 2 (2nd April 2020)

• Record Type: Journal Article
• Authors: Wang, Han-Cheng; Guo, Hua; Cai, Lin; Cai, Liu-Ti; Guo, Yu-Shuang; Ding, Wei
• Is Part Of: Canadian journal of plant pathology. Volume 42:Issue 2(2020)
• Page Start: 164
• Page End: 181
• Publication Date: 2020-04-02
• Subjects: metabolic phenotype -- phenomics -- Ralstonia solanacearum -- temperature -- tobacco bacterial wilt; flétrissure bactérienne du tabac -- phénomique -- phénotype métabolique -- Ralstonia solanacearum -- température; Plant diseases -- Canada -- Periodicals; Plant diseases -- Periodicals; 632.05
• DOI: 10.1080/07060661.2019.1654547
• Languages: English
• ISSNs: 0706-0661

Abstract

Abstract: The incidence of tobacco bacterial wilt, caused by Ralstonia solanacearum, is significantly affected by temperature. However, the effect of temperature on the metabolism of carbon and amino acid substrates used in phenotypic characterization of R. solanacearum is still unknown. In order to evaluate the effect of temperature on metabolic phenotypes, the phenotypes of the pathogen were characterized and compared with a BIOLOG Phenotype MicroArray (PM) at 20, 24, 30 and 35°C. The results showed that phenotypes of R. solanacearum at all four temperatures were mostly similar. However, a few differences were observed, such as in the ability to metabolize L-proline. The bacterium was able to metabolize more than 30% of the tested carbon sources and 83% of amino acid nitrogen substrates. For carbon sources, the most informative utilization patterns were organic acids and carbohydrates; for nitrogen sources, the most informative were amino acids. The bacterium showed much higher metabolic ability at 35°C than at 20, 24 and 30°C, and was more adaptable to osmolytes and pH environments at 30 and 35°C than at 20 and 24°C. It presented no deaminase activity, had high decarboxylase activity at 35°C and low decarboxylase activity at 20 and 24°C. When the phenotypic data were subjected to comparative phenomic analyses, temperature did have a significant effect on the characterization of the pathogen. The results provide clues to possible new methods to manage tobacco bacterial wilt.