Cell wall/vacuolar inhibitor of fructosidase 1 regulates ABA response and salt tolerance in Arabidopsis. (2nd April 2020)

• Record Type: Journal Article
• Authors: Yang, Wenting; Chen, Siyu; Cheng, Yuxin; Zhang, Na; Ma, Yanxing; Wang, Wei; Tian, Hainan; Li, Yingying; Hussain, Saddam; Wang, Shucai
• Is Part Of: Plant signaling & behavior. Volume 15:Number 4(2020)
• Publication Date: 2020-04-02
• Subjects: Cell wall/vacuolar inhibitor of fructosidase 1 -- abscisic acid -- salt tolerance -- gene editing -- Arabidopsis; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2020.1744293
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: ABA regulates abiotic stress tolerance in plants via activating/repressing gene expression. However, the functions of many ABA response genes remained unknown. C/VIFs are proteinaceous inhibitors of the CWI and VI invertases. We report here the involvement of C/VIF1 in regulating ABA response and salt tolerance in Arabidopsis. We found that the expression level of C/VIF1 was increased in response to ABA treatment. By using CRISPR/Cas9 gene editing, we generated transgene-free c/vif1 mutants. We also generated C/VIF1 overexpression plants by expressing C/VIF1 under the control of the 35S promoter. We examined ABA response of the 35S:C/VIF1 transgenic plants and the c/vif1 mutants by using seed germination and seedling greening assays, and found that the 35S:C/VIF1 transgenic plants showed an enhanced sensitivity to ABA treatment in both assays. On the other hand, the c/vif1 mutants showed slight enhanced tolerance to ABA only at the early stage of germination. We also found that salt tolerance was reduced in the 35S:C/VIF1 transgenic plants in seed germination assays, but slightly increased in the c/vif1 mutants. Taken together, our results suggest that C/VIF1 is an ABA response gene, and C/VIF1 is involved in the regulation of ABA response and salt tolerance in Arabidopsis.