Expression of rice siR109944 in Arabidopsis affects plant immunity to multiple fungal pathogens. (2nd April 2020)

• Record Type: Journal Article
• Authors: Qiao, Lulu; Zhao, Hongwei; Jin, Hailing; Niu, Dongdong
• Is Part Of: Plant signaling & behavior. Volume 15:Number 4(2020)
• Publication Date: 2020-04-02
• Subjects: Heterologous expression -- siRNA -- Arabidopsis immunity -- multiple fungal pathogens; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2020.1744347
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: Plant small RNA (sRNA)-mediated gene expression has a conserved role in regulating plant growth, development, and immunity. Heterologous expression of sRNA contributes to determining whether the function of sRNA is conservative or independent. We recently characterized the Tourist-miniature inverted-repeat transposable element (MITE)-derived siR109944 had a conserved function that enhanced susceptibility to Rhizoctonia solani infection by affecting auxin homeostasis in rice and Arabidopsis . To ascertain whether the function of rice siR109944 has a broad-spectrum immunity in Arabidopsis, we infected Arabidopsis with a variety of fungal pathogens. Overexpression of siR109944 in Arabidopsis increased susceptibility to Botrytis cinerea, Sclerotinia sclerotium, and Verticillium dahliae infection. Further studies found that Arabidopsis auxin-related miRNAs were suppressed in siR109944 OE . Our results demonstrated that overexpression of rice siR109944 in Arabidopsis affected immune responses to multiple pathogens by inhibiting auxin-related miRNA expression in planta .