Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor. Issue 16 (15th August 2020)

• Record Type: Journal Article
• Authors: Park, Myung Rae; Nam, Donggyu; Lee, Hyunah; Chang, Young-Tae; Zaehres, Holm; Kim, Jeong Beom
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 30:Issue 16(2020)
• Publication Date: 2020-08-15
• Subjects: DOFLA -- BODIPY -- Sensor -- Pluripotency -- Human pluripotent stem cells; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2020.127347
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: Human pluripotent stem cells (hPSCs) are a useful cell source for regenerative medicine. Despite having a potential of hPSCs for cell-based therapy, there is a need for a selective human pluripotency sensor for monitoring of live hPSCs. Here, we report the discovery of a novel pluripotency sensor (SHI5) from BODIPY-based library by high-throughput cell-based screening and describe the use of SHI5 to identify and isolate human embryonic stem cells and human induced pluripotent stem cells. We demonstrate that SHI5-based assay can be applied to live cells that gain pluripotency in the reprogramming process without any effect on their viability. We also show that SHI5 is internalized through a clathrin-mediated endocytosis pathway. These findings suggest that SHI5 can be an attractive sensor for pluripotency cells during reprogramming. Taken together, SHI5-based screening for hPSCs opens probably unlimited possibilities of detection probe for hPSC therapy via assures their safety issue.