Determination of phenolic compounds, in vitro antioxidant activity and characterization of secondary metabolites in different parts of Passiflora cincinnata by HPLC-DAD-MS/MS analysis. Issue 7 (2nd April 2020)

• Record Type: Journal Article
• Authors: Leal, Ana Ediléia Barbosa Pereira; de Oliveira, Ana Paula; Santos, Raira Feitosa dos; Soares, Juliana Mikaelly Dias; Lavor, Erica Martins de; Pontes, Michelle Cruz; Lima, Julianeli Tolentino de; Santos, Alan Diego da Conceição; Tomaz, José Carlos; Oliveira, Gibson Gomes de; Neto, Fausto Carnevale; Lopes, Norberto Peporine; Rolim, Larissa Araújo; Almeida, Jackson Roberto Guedes da Silva
• Is Part Of: Natural product research. Volume 34:Issue 7(2020)
• Page Start: 995
• Page End: 1001
• Publication Date: 2020-04-02
• Subjects: Antioxidant -- flavonoids -- Passiflora cincinnata -- phenolic compounds -- vitexin; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1548445
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Ethanol extracts of different parts of P assiflora cincinnata were obtained by maceration. The total phenolic and flavonoid contents were evaluated. The antioxidant activities were determined by β-carotene-linoleic acid bleaching test, 2, 2-diphenyl-1-picrylhydrazil (DPPH), and 2, 2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging. The crude ethanol stem extract showed the highest amount of total polyphenols (45.53 mg gallic acid equivalent/g) while the highest total flavonoid contents (1.42 mg of quercetin equivalent/g) were observed in the leaf extract. The lowest IC50 (25.65 μg/ml) by the DPPH method was observed for the stem extract. The ABTS method showed a significant antioxidant activity for all investigated extracts. The secondary metabolite composition of ethanol extracts was assessed by HPLC-DAD-MS/MS analysis, leading to the identification of fourteen secondary metabolites in P. cincinnata extracts. These results showed the potentiality of this species as a source of phenolic compounds and antioxidants. Graphical Abstract: