Anti-inflammatory activity of saponins from roots of Impatiens parviflora DC. Issue 11 (2nd June 2020)

• Record Type: Journal Article
• Authors: Grabowska, Karolina; Wróbel, Dagmara; Żmudzki, Paweł; Podolak, Irma
• Is Part Of: Natural product research. Volume 34:Issue 11(2020)
• Page Start: 1581
• Page End: 1585
• Publication Date: 2020-06-02
• Subjects: Saponins -- impatiens -- anti-denaturation activity -- hyaluronidase inhibition -- anti-inflammatory; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1519708
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Two triterpene saponins (IPS-1, IPS-2) for the first time were isolated from the roots of Impatiens parviflora DC. ( Balsaminaceae ). Their anti-inflammatory activity was evaluated by means of two in vitro models: anti-hyaluronidase and anti-denaturation assays. Both saponins were shown to be potent hyaluronidase inhibitors that affect the enzyme in a dose-dependent manner. The anti-hyaluronidase effect of IPS-2 (IC50 = 286.7 µg/mL) was higher than that of the reference drug: escin (IC50 = 303.93 µg/mL). Both saponins protected bovine serum albumin from heat-induced denaturation in a dose-dependent manner. IPS-1 demonstrated higher anti-denaturation effect (IC50 = 86.7 µg/ml) than IPS-2 (IC50 = 109.76 µg/mL) or the standard drug: acetylsalicylic acid (IC50 = 262.22 µg/mL). In conclusion, potent activity of IPS-1, IPS-2 in both in vitro assays shows that saponins from I. parviflora have anti-inflammatory activity. The obtained results allow to suggest that such compounds may be beneficial in inflammatory conditions, especially associated with excessive degradation of hyaluronic acid. Graphical Abstract: