Chemical Markers for Short- and Long-Term Areca Nut Exposure. (1st June 2020)
- Record Type:
- Journal Article
- Title:
- Chemical Markers for Short- and Long-Term Areca Nut Exposure. (1st June 2020)
- Main Title:
- Chemical Markers for Short- and Long-Term Areca Nut Exposure
- Authors:
- Franke, Adrian A.
Li, Xingnan
Custer, Laurie J.
Lai, Jennifer F. - Abstract:
- Abstract: Background: Areca nut (AN) chewing causes oral cancer. AN cessation programs are the most effective approach to reduce AN chewing induced cancers but require biomarkers to determine program compliance and success. Objectives: To explore chemical markers for short- and long-term AN exposure using non-invasively collected saliva, buccal cells (BCs), and scalp hair of chewers. Methods: Saliva was collected from a male chewer before and up to 2 days after AN chewing. Saliva was separated into supernatant and pellet (BCs) then analyzed by spectrophotometry and liquid chromatography (LC) with UV/VIS detection. Scalp hair was collected from four chewers and analyzed for areca alkaloids using direct analysis in real time-tandem mass spectrometry (DART-MSMS). Results: The red pigmented saliva after chewing showed no valuable signals when either the saliva supernatant or pellet (BCs) were analyzed by spectrophotometry. Saliva analysis by LC–UV/VIS showed diagnostically valuable signals at 488 nm up to 5 and 24 h post chewing in the supernatant and pellet, respectively. DART-MSMS analysis detected two of the four AN specific alkaloids (arecoline and arecaidine) in male but none in female hair. Conclusions/Importance: LC–UV/VIS analysis of the red pigments extracted from saliva and BCs after AN chewing showed distinct signals up to 24 h post chewing while DART-MSMS analysis in BCs and scalp hair showed selective signals of AN alkaloids for several weeks or months after ANAbstract: Background: Areca nut (AN) chewing causes oral cancer. AN cessation programs are the most effective approach to reduce AN chewing induced cancers but require biomarkers to determine program compliance and success. Objectives: To explore chemical markers for short- and long-term AN exposure using non-invasively collected saliva, buccal cells (BCs), and scalp hair of chewers. Methods: Saliva was collected from a male chewer before and up to 2 days after AN chewing. Saliva was separated into supernatant and pellet (BCs) then analyzed by spectrophotometry and liquid chromatography (LC) with UV/VIS detection. Scalp hair was collected from four chewers and analyzed for areca alkaloids using direct analysis in real time-tandem mass spectrometry (DART-MSMS). Results: The red pigmented saliva after chewing showed no valuable signals when either the saliva supernatant or pellet (BCs) were analyzed by spectrophotometry. Saliva analysis by LC–UV/VIS showed diagnostically valuable signals at 488 nm up to 5 and 24 h post chewing in the supernatant and pellet, respectively. DART-MSMS analysis detected two of the four AN specific alkaloids (arecoline and arecaidine) in male but none in female hair. Conclusions/Importance: LC–UV/VIS analysis of the red pigments extracted from saliva and BCs after AN chewing showed distinct signals up to 24 h post chewing while DART-MSMS analysis in BCs and scalp hair showed selective signals of AN alkaloids for several weeks or months after AN exposure. Chemical hair treatment might prevent detection of areca alkaloids in hair. AN cessation trials and other programs now have essential tools for bioverification. … (more)
- Is Part Of:
- Substance use & misuse. Volume 55:Number 9(2020)
- Journal:
- Substance use & misuse
- Issue:
- Volume 55:Number 9(2020)
- Issue Display:
- Volume 55, Issue 9 (2020)
- Year:
- 2020
- Volume:
- 55
- Issue:
- 9
- Issue Sort Value:
- 2020-0055-0009-0000
- Page Start:
- 1395
- Page End:
- 1402
- Publication Date:
- 2020-06-01
- Subjects:
- Areca nut -- saliva -- buccal cells -- scalp hair -- biomarkers -- cessation program -- HPLC -- UV/VIS spectrophotometry -- DART-MS
Narcotic habit -- Periodicals
Alcoholism -- Periodicals
Substance abuse -- Periodicals
Behavior, Addictive -- Periodicals
Sustance-Related Disorders -- Periodicals
362.2905 - Journal URLs:
- http://informahealthcare.com/loi/sum ↗
http://informahealthcare.com ↗ - DOI:
- 10.1080/10826084.2019.1630442 ↗
- Languages:
- English
- ISSNs:
- 1082-6084
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 8503.493000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 13623.xml