Endothelial RhoB and RhoC are dispensable for leukocyte diapedesis and for maintaining vascular integrity during diapedesis. (3rd May 2020)

• Record Type: Journal Article
• Authors: Schimmel, Lilian; de Ligt, Aafke; Tol, Simon; de Waard, Vivian; van Buul, Jaap D.
• Is Part Of: Small GTPases. Volume 11:Number 3(2020)
• Page Start: 225
• Page End: 232
• Publication Date: 2020-05-03
• Subjects: endothelial -- permeability -- RhoB -- RhoC -- transmigration -- vascular leakage; Guanosine triphosphatase -- Periodicals; Guanosine triphosphatase; Periodicals; 572.793
• DOI: 10.1080/21541248.2017.1377815
• Languages: English
• ISSNs: 2154-1256

Abstract

ABSTRACT: Active remodeling of the actin cytoskeleton in endothelial cells is necessary for allowing leukocytes to cross the barrier during the process of transendothelial migration (TEM). Involvement of RhoGTPases to regulate actin organization is inevitable, and we recently reported on the local function of RhoA in limiting vascular leakage during leukocyte TEM. As a follow-up we investigated here the possible involvement of two other closely-related GTPases; RhoB and RhoC, in regulating leukocyte TEM and vascular barrier maintenance. Physiological flow experiments showed no substantial involvement of either endothelial RhoB or RhoC in neutrophil adhesion and transmigration efficiency. Besides neutrophil TEM, we did not observe a role for endothelial RhoB or RhoC in limiting vascular leakage in both inflammatory conditions and during TEM. In conclusion, endothelial RhoB and RhoC are both dispensable for regulating leukocyte diapedesis and for maintaining vascular barrier function under inflammatory conditions and during leukocyte diapedesis.