Methods to Measure MDSC Immune Suppressive Activity In Vitro and In Vivo. (10th October 2018)

• Record Type: Journal Article
• Authors: Solito, Samantha; Pinton, Laura; De Sanctis, Francesco; Ugel, Stefano; Bronte, Vincenzo; Mandruzzato, Susanna; Marigo, Ilaria
• Editors: Coligan, John E.; Dunn, Ben M.; Speicher, David W.; Wingfield, Paul T.
• Is Part Of: Current protocols in immunology. Volume 124(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-10
• Subjects: adoptive cell transfer -- chromium release test -- flow cytometry -- immunomagnetic sorting -- immunosuppression -- MDSC -- thymidine incorporation; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/cpim.61
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: This unit presents methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid‐derived suppressor cells (MDSCs), both in vitr o and in vivo in mice, as well as in biological samples from cancer patients. These methods could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover, they could be useful to assess the influence exerted by genetic modifications, chemical inhibitors, and drugs on immune suppressive pathways © 2018 by John Wiley & Sons, Inc.