Auxiliary voltage enhanced microbial methane oxidation co-driven by nitrite and sulfate reduction. (July 2020)

• Record Type: Journal Article
• Authors: Chai, Fengguang; Li, Lin; Xue, Song; Liu, Junxin
• Is Part Of: Chemosphere. Volume 250(2020)
• Publication Date: 2020-07
• Subjects: Methane oxidation -- Nitrite reduction -- Sulfate reduction -- Auxiliary voltage -- Microbial population -- Potential pathway; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.126259
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: In this study, single-chamber bioelectrochemical reactors (EMNS) were used to investigate the methane oxidation driven by sulfate and nitrite reduction with the auxiliary voltage. Results showed that the methane oxidation was simultaneously driven by sulfate and nitrite reduction, with more methane being converted using the auxiliary voltage. When the voltage was 1.6 V, the maximum removal rate was achieved at 8.05 mg L −1 d −1 . Carbon dioxide and methanol were the main products of methane oxidation. Simultaneously, nitrogen, nitrous oxide, sulfur ions, and hydrogen sulfide were detected as products of sulfate and nitrite reduction. Microbial populations were analyzed by qPCR and high-throughput sequencing. The detected methanotrophs included Methylocaldum sp., Methylocystis sp., Methylobacter sp. and M. oxyfera . The highest abundance of M. oxyfera was (3.97 ± 0.32) × 10 6 copies L −1 in the EMNS-1.6. The dominant nitrite-reducing bacteria were Ignavibacterium sp., Hyphomicrobium sp., Alicycliphilus sp., and Anammox bacteria. Desulfovibrio sp., Desulfosporosinu s sp. and Thiobacillus sp. were related to the sulfur cycle . Ignavibacterium sp., Thiobacillus sp. and Desulfovibrio sp. may transfer electrons with electrodes using humic acids as the electronic shuttle. The possible pathways included (1) Methane was mainly oxidized to carbon dioxide and dissolved organic matters by methanotrophs utilizing the oxygen produced by the disproportionation in the cells of M. oxyfera . (2) Nitrite was reduced to nitrogen by heterotrophic denitrifying bacteria with dissolved organic compounds. (3) Desulfovibrio sp. and Desulfosporosinu s sp. reduced sulfate to sulfur ions. Thiobacillus sp. oxidized sulfur ions to sulfur or sulfate using nitrite as the electron acceptor. Graphical abstract: Image 1 Highlights: Methane oxidation can be co-driven by nitrite and sulfate reduction. Auxiliary voltage plays an important role in strengthening methane oxidation. The conversion products of methane were carbon dioxide and methanol. The dominant methanotrophs included the M. oxyfera, Methylocystis sp., Methylobacter sp., and Methylocaldum sp.. Possible pathways were proposed for the methane oxidation coupled with nitrite and sulfate reduction.