Mosaic genome of Human Coxsackievirus A4 associated with herpangina and HFMD in Yancheng, China, 2016 and 2018. (July 2020)

• Record Type: Journal Article
• Authors: Guo, Wen-Ping; Chen, Guo-Qing; Xie, Guang-Cheng; Du, Luan-Ying; Tang, Quan
• Is Part Of: International journal of infectious diseases. Volume 96(2020)
• Page Start: 538
• Page End: 540
• Publication Date: 2020-07
• Subjects: coxckievirus A4 -- hand foot and mouth disease -- herpangina -- recombination; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2020.05.057
• Languages: English
• ISSNs: 1201-9712

Abstract

Highlights: CV-A4 was associated with the major herpangina cases in Yancheng, 2018. One novel CV-A4 group was identified in HFMD cases. P2 and P3 regions of all 7 strains in this study clustered together. Abstract: Objectives: To better understand the spectrums of pathogens causing herpangina and circulation of Coxsackievirus A4 in Yancheng, China. Methods: Stool samples from herpangina and HFMD cases were collected. Real Time PCR Kits was used to identify Enterovirus 71, CV-A16 and CV-A6, and nested reverse transcription PCR (nRT-PCR) to detect the other enterovirus types. Complete VP1 and genome sequence of CV-A4 were amplified by using nRT-PCR. Genetic, phylogenetic and recombination analysis were performed. Results: Co-circulation of three recombinant CV-A4 groups, including one novel (C2 lineage), was identified in Yancheng, China, 2016 and 2018. One was the major causative agent of herpangina, and another two were responsible for HFMD. Phylogenetic and recombination analysis indicated that the non-structural region of their genome originated from the same ancestry and subsequently adaptation. C2 lineage of CV-A4 group may be introduced from countries outside China and its genome occurred recombination in China. Conclusion: Novel recombinant CV-A4 was mainly associated with herpanginain in Yancheng, 2018, China. C2 lineage of CV-A4 group with recombinant non-structural region was also identified in HFMD patients.