YB-3 substitutes YB-1 in global mRNA binding. Issue 4 (2nd April 2020)

• Record Type: Journal Article
• Authors: Lyabin, D. N.; Eliseeva, I. A.; Smolin, E. A.; Doronin, A. N.; Budkina, K. S.; Kulakovskiy, I. V.; Ovchinnikov, L. P.
• Is Part Of: RNA biology. Volume 17:Issue 4(2020)
• Page Start: 487
• Page End: 499
• Publication Date: 2020-04-02
• Subjects: YB-1 -- YBX1 -- YB-3 -- YBX3 -- Ribo-Seq -- RIP-Seq -- translational control; RNA -- Periodicals; Molecular biology -- Periodicals; Molecular biology; RNA; Periodicals; 572.8805
• DOI: 10.1080/15476286.2019.1710050
• Languages: English
• ISSNs: 1547-6286

Abstract

ABSTRACT: Y-box binding proteins are DNA- and RNA-binding proteins with an evolutionarily ancient and conserved cold shock domain. The Y-box binding protein 1 (YB-1) is the most studied due to its abundance in somatic cells. YB-1 is involved in a variety of cellular processes, including proliferation, differentiation and stress response. Here, using Ribo-Seq and RIP-Seq we confirm that YB-1 binds a wide range of mRNAs and globally acts as a translation inhibitor. Surprisingly, YBX1 knockout results in only minor alterations in the expression of other genes, mostly caused by changes in RNA abundance. But YB-3 mRNA is an exception: it is better translated in the absence of YB-1, thereby producing an increased amount of YB-3 and thus suggesting that its synthesis is under YB-1 negative control. We have shown that the set of mRNAs bound to YB-3 is strikingly similar to that of YB-1, and that the mRNA-binding by YB-3 is enhanced in the absence of YB-1, resulting in a similar global reduction of translation of bound mRNAs in YB-1-null cells. Thus, YB-3 acts as a substitute for YB-1 in mRNA binding and, probably, in global translational control.