Administration of branched‐chain amino acids alters the balance between pro‐inflammatory and anti‐inflammatory cytokines. Issue 48 (30th November 2015)

• Record Type: Journal Article
• Authors: Rosa, Luciana; Scaini, Giselli; Furlanetto, Camila B.; Galant, Leticia S.; Vuolo, Francieli; Dall'Igna, Dhébora M.; Schuck, Patrícia F.; Ferreira, Gustavo C.; Dal‐Pizzol, Felipe; Streck, Emilio L.
• Is Part Of: International journal of developmental neuroscience. Issue 48(2016:Feb.)
• Page Start: 24
• Page End: 30
• Publication Date: 2015-11-30
• Subjects: Branched chain amino acids -- Maple syrup urine disease -- Brain -- Cytokines; Developmental neurobiology -- Periodicals; Neurology -- Periodicals; Neurologie du développement -- Périodiques; Developmental neurobiology; Periodicals; 612.8
• DOI: 10.1016/j.ijdevneu.2015.11.002
• Languages: English
• ISSNs: 0736-5748

Abstract

Abstract: Acute leucine intoxication and neurologic deterioration can develop rapidly at any age as a result of net protein degradation precipitated by infection or psychological stress in patients with maple syrup urine disease (MSUD). Here, we investigated the effects of acute and chronic Hyper‐BCAA (H‐BCAA) administration on pro‐ and anti‐inflammatory cytokines in the brains of rats. For acute administration, Wistar rats (10 and 30 days) received three injections of BCAA pool (15.8 μL/g at 1‐h intervals) or saline, subcutaneously. For chronic administration, Wistar rats (7 days) received of BCAA pool or saline twice a day for 21 days, subcutaneously. Our results showed that acute administration of H‐BCAA increased IL‐1β (∼78%; p ≤ 0.009) and TNF‐α (∼155%; p ≤ 0.026) levels in the cerebral cortex but not in the hippocampus of infant rats. Moreover, IL‐6 levels were increased in the hippocampus (∼135%; p ≤ 0.009) and cerebral cortex (∼417%; p ≤ 0.008), whereas IL‐10 levels were decreased only in the hippocampus (∼42%; p ≤ 0.009). However, repeated administration of H‐BCAA decreased IL‐1β (∼59%; p ≤ 0.047), IL‐6 (∼70%; p ≤ 0.009) and IFN‐γ (∼70%; p ≤ 0.008) levels in the cerebral cortex, whereas the IL‐6 (∼67%; p ≤ 0.009), IL‐10 (∼58%; p ≤ 0.01) and IFN‐γ (∼67%; p ≤ 0.009) levels were decreased in the hippocampus. These findings suggest that a better understanding of the inflammatory response in MSUD patients may be useful to develop therapeutic strategies to modulate the hyperinflammatory/hypoinflammatory axis.