Artificial Restriction DNA Cutter Using Nuclease S1 for Site‐Selective Scission of Genomic DNA. Issue 1 (5th February 2019)

• Record Type: Journal Article
• Authors: Rajendran, Arivazhagan; Shigi, Narumi; Sumaoka, Jun; Komiyama, Makoto
• Editors: Beaucage, Serge L.; Bergstrom, Donald E.; Herdewijn, Piet; Matsuda, Akira
• Is Part Of: Current protocols in nucleic acid chemistry. Volume 76:Issue 1(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-02-05
• Subjects: artificial restriction DNA cutter -- blue fluorescent protein gene -- human genome -- peptide nucleic acids -- S1 nuclease -- telomere DNA; Nucleic acids -- Laboratory manuals; Nucleic Acids -- chemical synthesis; Nucleic acids; Laboratory Manuals; Electronic books; Laboratory manuals; 572.8028
• DOI: 10.1002/cpnc.72
• Languages: English
• ISSNs: 1934-9270

Abstract

Abstract: By combining a pair of pseudo‐complementary peptide nucleic acids (pcPNAs) with S1 nuclease, a novel tool to cut DNA at a predetermined site can be obtained. Complementary pcPNAs invade the DNA duplex and base pair to each strand of a target site, creating single‐stranded regions that are cleaved by S1 nuclease. The scission site can be freely modulated by the design of pcPNAs. This method can be used to cleave a single site in the human genome. This protocol presents experimental details for site‐selective scission using this versatile new tool. © 2019 by John Wiley & Sons, Inc.