Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2. Issue 4 (17th October 2018)

• Record Type: Journal Article
• Authors: Nelson‐Vasilchik, Kimberly; Hague, Joel; Mookkan, Muruganantham; Zhang, Zhanyuan J.; Kausch, Albert
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 3:Issue 4(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-17
• Subjects: Agrobacterium tumefaciens -- morphogenic regulators -- Sorghum bicolor -- sorghum transformation; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20076
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Most reliable transformation protocols for cereal crops, including sorghum ( Sorghum bicolor L. Moench), rely on the use of immature embryo explants to generate embryogenic callus cells that are then transformed using Agrobacterium ‐ or particle‐bombardment‐mediated DNA delivery. Subsequent to DNA transfer, most protocols rely on selectable markers for the recovery of stably transformed callus that is then regenerated to produce T0 plants. However, these protocols require specific genotypes that are innately capable of efficient embryogenic callus initiation. Here, we describe a system that makes use of the differential expression of the morphogenic regulators Baby Boom ( Bbm ) and Wuschel2 ( Wus2 ) to achieve transformation in varieties of sorghum typically recalcitrant to standard transformation methods. © 2018 by John Wiley & Sons, Inc.