UNIT 9.5 Objective Morphological Quantification of Microscopic Images Using a Fast Fourier Transform (FFT) Analysis. Issue 7 (23rd October 2013)

• Record Type: Journal Article
• Authors: Taylor, Samuel E.; Cao, Tuoxin; Talauliker, Pooja M.; Lifshitz, Jonathan
• Is Part Of: Current protocols essential laboratory techniques. Issue 7(2014)
• Page Start: 9.5.1
• Page End: 9.5.12
• Publication Date: 2013-10-23
• Subjects: quantification -- microscopy -- fast Fourier transformation -- immunohistochemistry; Life sciences -- Laboratory manuals; Medical laboratories; Diagnosis, Laboratory; Clinical Laboratory Techniques; Clinical Protocols; Diagnosis, Laboratory; Medical laboratories; 570.28
• DOI: 10.1002/9780470089941.et0905s07
• Languages: English
• ISSNs: 1948-3430

Abstract

Abstract: Quantification of immunohistochemistry (IHC) and immunofluorescence (IF) using image intensity depends on a number of variables. These variables add a subjective complexity in keeping a standard within and between laboratories. Fast Fourier Transformation (FFT) algorithms, however, allow for a rapid and objective quantification (via statistical analysis) using cell morphologies when the microscopic structures are oriented or aligned. Quantification of alignment is given in terms of a ratio of FFT intensity to the intensity of an orthogonal angle, giving a numerical value of the alignment of the microscopic structures. This allows for a more objective analysis than alternative approaches, which rely upon relative intensities. Curr. Protoc. Essential Lab. Tech . 7:9.5.1‐9.5.12. © 2013 by John Wiley & Sons, Inc.