Proximity Ligation Assay (PLA). (20th September 2018)

• Record Type: Journal Article
• Authors: Alam, Muhammad S.
• Editors: Coligan, John E.; Bierer, Barbara E.; Margulies, David H.; Shevach, Ethan M.; Strober, Warren
• Is Part Of: Current protocols in immunology. Volume 123(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-09-20
• Subjects: antibody -- Duolink® -- in situ assays -- protein‐protein interaction; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/cpim.58
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: Proximity ligation assay (PLA), also referred to as Duolink® PLA technology, permits detection of protein‐protein interactions in situ (at distances <40 nm) at endogenous protein levels. It exploits specific antibodies identifying (either directly or indirectly) the two proteins of interest and utilizes specific DNA primers covalently linked to the antibodies. A hybridization step followed by DNA amplification with fluorescent probes permit visualization of spots of proximity by fluorescence microscopy. Since the development of PLA in 2002, it has been increasingly used to detect the interaction between two proteins with high sensitivity and specificity. It is a simple and sensitive technique to study protein‐protein interaction in cells. © 2018 by John Wiley & Sons, Inc.