Engineering Bacterial Shape Using Soft Matter Microchambers. Issue 1 (7th December 2018)

• Record Type: Journal Article
• Authors: Renner, Lars David
• Editors: Arkin, Adam P.; Mahal, Lara; Romesberg, Floyd; Shah, Kavita; Shamu, Caroline; Thomas, Craig
• Is Part Of: Current protocols in chemical biology. Volume 11:Issue 1(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-12-07
• Subjects: bacteria -- mechanics -- microbiology -- microfabrication -- spheroplasts; Biochemistry -- Laboratory manuals; Chemical Phenomena; Biochemistry; Periodicals; Laboratory manuals; 572.078
• DOI: 10.1002/cpch.59
• Languages: English
• ISSNs: 2160-4762

Abstract

Abstract: This article describes the design and fabrication of microchambers that are used for the study of bacterial cells. The design allows for the confinement and precise manipulation of bacterial cell shape. The application of fluorescent dyes and fluorescent proteins enables the precise analysis of the localization of biomolecules within confined bacterial cell. This article also outlines three methods to engineer cell shape from a filamentous cell type and from spheroplasts without a cell wall using soft lithography–based technologies. © 2018 by John Wiley & Sons, Inc.