Aseptic Technique. Issue 18 (16th April 2019)

• Record Type: Journal Article
• Authors: Bykowski, Tomasz; Holt, Jonathan F.; Stevenson, Brian
• Editors: Gallagher, Sean R.; Wiley, Emily A.
• Is Part Of: Current protocols essential laboratory techniques. Issue 18(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-04-16
• Subjects: biosafety cabinet -- bunsen burner -- laminar flow -- laminar hood -- sterile technique -- sterile work; Life sciences -- Laboratory manuals; Medical laboratories; Diagnosis, Laboratory; Clinical Laboratory Techniques; Clinical Protocols; Diagnosis, Laboratory; Medical laboratories; 570.28
• DOI: 10.1002/cpet.31
• Languages: English
• ISSNs: 1948-3430

Abstract

Abstract: This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as "aseptic technique." Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes. © 2019 by John Wiley & Sons, Inc.