Real‐Time Chemiluminescence Imaging Using Nano‐Lantern Probes. Issue 4 (1st December 2014)

• Record Type: Journal Article
• Authors: Arai, Yoshiyuki; Nagai, Takeharu
• Editors: Mahal, Lara; Romesberg, Floyd; Shah, Kavita; Shamu, Caroline; Strano, Michael S.; Thomas, Craig
• Is Part Of: Current protocols in chemical biology. Volume 6:Issue 4(2014)
• Page Start: 221
• Page End: 236
• Publication Date: 2014-12-01
• Subjects: Nano‐lantern -- Nano‐lantern (Ca2+) -- optogenetics -- channelrhodopsin2 (ChR2) -- dead time -- CCD camera; Biochemistry -- Laboratory manuals; Chemical Phenomena; Biochemistry; Periodicals; Laboratory manuals; 572.078
• DOI: 10.1002/9780470559277.ch140168
• Languages: English
• ISSNs: 2160-4762

Abstract

Abstract: Chemiluminescence imaging can be performed without excitation light sources at various spatial levels ranging from a single cell to the whole body. Thus far, chemiluminescence imaging has been primarily performed with long exposure times because of weak signals, resulting in low temporal resolution. Recently, the brightest‐known chemiluminescent proteins—Nano‐lantern and Nano‐lantern‐based functional indicators—have been developed. Nano‐lantern probes break the limitation of temporal resolution and enable chemiluminescence imaging of living samples such as cells, plants, and small animals at video rates. This unit describes one protocol for observation of a freely moving unshaved mouse transplanted with Nano‐lantern‐expressing tumor cells, and another for compatible use of optogenetic tools and a Nano‐lantern calcium indicator. Both protocols utilize the synchronization of illumination and camera acquisition sessions, thereby enabling real‐time chemiluminescence imaging. © 2014 by John Wiley & Sons, Inc.