Pseudo‐Ligandless Click Chemistry for Oligonucleotide Conjugation. Issue 2 (2nd June 2016)

• Record Type: Journal Article
• Authors: Mack, Stephanie; Fouz, Munira F.; Dey, Sourav K.; Das, Subha R.
• Editors: Mahal, Lara; Romesberg, Floyd; Shah, Kavita; Shamu, Caroline; Strano, Michael S.; Thomas, Craig
• Is Part Of: Current protocols in chemical biology. Volume 8:Issue 2(2016)
• Page Start: 83
• Page End: 95
• Publication Date: 2016-06-02
• Subjects: alkyne -- azide -- bioconjugation -- DNA -- labeling -- nucleic acids -- RNA; Biochemistry -- Laboratory manuals; Chemical Phenomena; Biochemistry; Periodicals; Laboratory manuals; 572.078
• DOI: 10.1002/cpch.1
• Languages: English
• ISSNs: 2160-4762

Abstract

Abstract: Particularly for its use in bioconjugations, the copper‐catalyzed (or copper‐promoted) azide‐alkyne cycloaddition (CuAAC) reaction or 'click chemistry', has become an essential component of the modern chemical biologist's toolbox. Click chemistry has been applied to DNA, and more recently, RNA conjugations, and the protocols presented here can be used for either. The reaction can be carried out in aqueous buffer, and uses acetonitrile as a minor co‐solvent that serves as a ligand to stabilize the copper. The method also includes details on the analysis of the reaction product. © 2016 by John Wiley & Sons, Inc.