Generating FN3‐Based Affinity Reagents Through Phage Display. Issue 2 (7th June 2018)

• Record Type: Journal Article
• Authors: Gorman, Kevin; McGinnis, Jennifer; Kay, Brian
• Editors: Arkin, Adam P.; Mahal, Lara; Romesberg, Floyd; Shah, Kavita; Shamu, Caroline; Thomas, Craig
• Is Part Of: Current protocols in chemical biology. Volume 10:Issue 2(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-06-07
• Subjects: affinity -- immunassay -- monobody -- phage display -- purification; Biochemistry -- Laboratory manuals; Chemical Phenomena; Biochemistry; Periodicals; Laboratory manuals; 572.078
• DOI: 10.1002/cpch.39
• Languages: English
• ISSNs: 2160-4762

Abstract

Abstract: Antibodies are useful tools for detecting individual proteins in complex samples and for learning about their location, amount, binding partners, and function in cells. Unfortunately, generating antibodies is time consuming and laborious, and their affinity and/or specificity is often limited. This protocol offers a fast and inexpensive alternative to generate antibody surrogates through phage display of a library of fibronectin type III (FN3) monobody variants and affinity selection for binders. © 2018 by John Wiley & Sons, Inc.