Absolute Quantitation of Plant Proteins. Issue 1 (2nd April 2018)

• Record Type: Journal Article
• Authors: Ahsan, Nagib; Wilson, Rashaun S.; Thelen, Jay J.
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian‐Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 3:Issue 1(2018)
• Page Start: 1
• Page End: 13
• Publication Date: 2018-04-02
• Subjects: AQUA peptide -- multiple reaction monitoring -- plant protein -- triple quadrupole mass spectrometer -- targeted proteomics; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20064
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Among targeted proteomic techniques, AQUA‐MRM is considered as one of the most reliable for accurate protein quantitation. This method displays high sensitivity, specificity, and reproducibility compared to many common biochemical techniques by coupling the use of unique, heavy‐labeled peptide standards and triple‐quadrupole mass spectrometry. However, there are several important steps that are required for successful development and validation of a robust AQUA‐MRM assay. The following protocol outlines and details the key steps necessary for plant sample preparation as well as AQUA‐MRM development and validation, specifically for absolute quantitation of plant proteins in vivo . © 2018 by John Wiley & Sons, Inc.