Highly Efficient Agrobacterium‐Mediated Transformation of Potato (Solanum tuberosum) and Production of Transgenic Microtubers. Issue 1 (2nd April 2018)

• Record Type: Journal Article
• Authors: Craze, Melanie; Bates, Ruth; Bowden, Sarah; Wallington, Emma J.
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian‐Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 3:Issue 1(2018)
• Page Start: 33
• Page End: 41
• Publication Date: 2018-04-02
• Subjects: agrobacterium‐mediated transformation -- in vitro cultures -- potato -- microtubers; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20065
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: The following method enables the rapid production of transgenic potato plants and microtubers for gene validation and expression, or promoter studies. The method is highly efficient, with reproducible transformation efficiencies of at least 50% to 60% with potato cv. Desiree, and can produce transgenic microtubers within 6 months of initiation of the experiment. Microtubers are produced in the absence of hormones, giving an in vitro gene testing system broadly analogous to the natural state. © 2018 by John Wiley & Sons, Inc.