UNIT 7.41 Sampling of Intestinal Microbiota and Targeted Amplification of Bacterial 16S rRNA Genes for Microbial Ecologic Analysis. (3rd November 2014)

• Record Type: Journal Article
• Authors: Tong, Maomeng; Jacobs, Jonathan P.; McHardy, Ian H.; Braun, Jonathan
• Is Part Of: Current protocols in immunology. Volume 107(2014)
• Page Start: 7.41.1
• Page End: 7.41.11
• Publication Date: 2014-11-03
• Subjects: intestinal microbiota -- mucosal microbiota -- lavage -- Illumina sequencing -- 16S rRNA; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/0471142735.im0741s107
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract : Dysbiosis of host‐associated commensal microbiota is emerging as an important factor in risk and phenotype of immunologic, metabolic, and behavioral diseases. Accurate analysis of microbial composition and functional state in humans or mice requires appropriate collection and pre‐processing of biospecimens. Methods to sample luminal and mucosal microbiota from human or mouse intestines and to profile microbial phylogenetic composition using 16S rRNA sequencing are presented here. Data generated using the methods in this unit can be used for downstream quantitative analysis of microbial ecology. © 2014 by John Wiley & Sons, Inc.