UNIT 9.10 Use of Proteasome Inhibitors. (1st April 2015)

• Record Type: Journal Article
• Authors: Downey, Sondra L.; Florea, Bogdan I.; Overkleeft, Herman S.; Kisselev, Alexei F.
• Is Part Of: Current protocols in immunology. Volume 109(2015)
• Page Start: 9.10.1
• Page End: 9.10.9
• Publication Date: 2015-04-01
• Subjects: proteosome inhibitors -- peptide aldehydes -- boronates -- peptide epoxyketones; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/0471142735.im0910s109
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract : Proteasome inhibitors are indispensable research tools in immunology and cell biology. With numerous proteasome inhibitors available commercially, choosing the appropriate compound for a biological experiment may be challenging, especially for a novice. This unit provides an overview of the proteasome inhibitors commonly used in research. It discusses how to select an appropriate highly specific inhibitor, its concentration, and length of exposure for mammalian cell culture experiments. In addition, assays that can be used to confirm proteasome inhibition are discussed. © 2015 by John Wiley & Sons, Inc.