UNIT 2.18 Oil‐Based Emulsion Vaccine Adjuvants. (1st August 2014)

• Record Type: Journal Article
• Authors: Schijns, Virgil E.J.C.; Strioga, Marius; Ascarateil, Stephane
• Is Part Of: Current protocols in immunology. Volume 106(2014)
• Page Start: 2.18.1
• Page End: 2.18.7
• Publication Date: 2014-08-01
• Subjects: emulsions -- vaccines -- adjuvants; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/0471142735.im0218s106
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: Vaccine adjuvants are critical components in experimental and licensed vaccines used in human and veterinary medicine. When aiming to evoke an immune response to a purified antigen, the administration of antigen alone is often insufficient, unless the antigen contains microbial structures or has a natural particulate structure. In most cases, the rationale to use an adjuvant is obvious to the experimental immunologist or the professional vaccinologist, who is familiar with the nature of the antigen, and the aim of the vaccine to elicit a specific antibody response and/or a specific type of T cell response. In this unit, we describe protocols to formulate antigens with oil‐based emulsions. Such emulsions represent a major prototype adjuvant category that is frequently used in experimental preclinical vaccines, as well as veterinary and human vaccines. Curr. Protoc. Immunol . 106:2.18.1‐2.18.7. © 2014 by John Wiley & Sons, Inc.