UNIT 3.22 Isolation of Murine Natural Killer Cells. (2nd April 2014)

• Record Type: Journal Article
• Authors: Pak‐Wittel, Melissa A.; Piersma, Sytse J.; Plougastel, Beatrice F.; Poursine‐Laurent, Jennifer; Yokoyama, Wayne M.
• Is Part Of: Current protocols in immunology. Volume 105(2014)
• Page Start: 3.22.1
• Page End: 3.22.9
• Publication Date: 2014-04-02
• Subjects: immunology -- innate immunity -- cell isolation -- magnetic separation; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/0471142735.im0322s105
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: This unit describes the isolation of natural killer (NK) cells from mouse spleen. The basic protocol describes a method for preparing a highly purified NK cell population from mouse spleen by depletion of contaminating cells with selected monoclonal antibodies (MAbs) and magnetic separation. There are several advantages to this negative selection process. One of these is that the NK cells are not coated with antibody and, therefore, are not at risk of functional perturbation by antibody cross‐linking. Additionally, negative selection provides a way to isolate diverse subpopulations of NK cells without selectively purifying a specific subpopulation. Following enrichment, NK cell purity can be assessed by cell surface phenotype using flow cytometry. Curr. Protoc. Immunol .. 105:3.22.1‐3.22.9. © 2014 by John Wiley & Sons, Inc.