Profiling Interactome Networks with the HaloTag‐NAPPA In Situ Protein Array. Issue 3 (14th August 2018)

• Record Type: Journal Article
• Authors: Yazaki, Junshi; Galli, Mary; Kim, Alice Y.; Ecker, Joseph R.
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 3:Issue 3(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-08-14
• Subjects: cell‐free protein expression -- HaloTag‐NAPPA -- in situ protein array -- interactome -- protein‐protein interaction; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20071
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Physical interactions between proteins and other molecules can be evaluated at a proteome scale using protein arrays, a type of high‐throughput pulldown assay. We developed a modified in situ protein array known as the nucleic acid programmable protein assay (NAPPA) that allows the screening of thousands of open reading frames (ORFs) at a lower cost, with less labor, and in less time than conventional protein arrays. The HaloTag‐NAPPA protein array can efficiently capture proteins expressed in situ on a glass slide using the Halo high‐affinity capture tag. Here, we describe the fabrication of the array using publicly available resources and detection of protein‐protein interactions (PPIs) that can be used to generate a protein interactome map. The Basic Protocol includes procedures for preparing the plasmid DNA spotted on glass slides, in situ protein expression, and PPI detection. The supporting protocols outline the construction of vectors and preparation of ORF clones. © 2018 by John Wiley & Sons, Inc.