Designing Drug‐Response Experiments and Quantifying their Results. Issue 2 (19th June 2017)

• Record Type: Journal Article
• Authors: Hafner, Marc; Niepel, Mario; Subramanian, Kartik; Sorger, Peter K.
• Is Part Of: Current protocols in chemical biology. Volume 9:Issue 2(2017)
• Page Start: 96
• Page End: 116
• Publication Date: 2017-06-19
• Subjects: experimental design -- drug response -- data processing -- computational pipeline; Biochemistry -- Laboratory manuals; Chemical Phenomena; Biochemistry; Periodicals; Laboratory manuals; 572.078
• DOI: 10.1002/cpch.19
• Languages: English
• ISSNs: 2160-4762

Abstract

Abstract : We developed a Python package to help in performing drug‐response experiments at medium and high throughput and evaluating sensitivity metrics from the resulting data. In this article, we describe the steps involved in (1) generating files necessary for treating cells with the HP D300 drug dispenser, by pin transfer or by manual pipetting; (2) merging the data generated by high‐throughput slide scanners, such as the Perkin Elmer Operetta, with treatment annotations; and (3) analyzing the results to obtain data normalized to untreated controls and sensitivity metrics such as IC50 or GR50 . These modules are available on GitHub and provide an automated pipeline for the design and analysis of high‐throughput drug response experiments, that helps to prevent errors that can arise from manually processing large data files. © 2017 by John Wiley & Sons, Inc.