Production and Thermal Exchange of Conditional Peptide‐MHC I Multimers. (26th July 2019)
- Record Type:
- Journal Article
- Title:
- Production and Thermal Exchange of Conditional Peptide‐MHC I Multimers. (26th July 2019)
- Main Title:
- Production and Thermal Exchange of Conditional Peptide‐MHC I Multimers
- Authors:
- Luimstra, Jolien J.
Franken, Kees L. M. C.
Garstka, Malgorzata A.
Drijfhout, Jan W.
Neefjes, Jacques
Ovaa, Huib - Editors:
- Coligan, John E.
Bierer, Barbara E.
Margulies, David H.
Shevach, Ethan M.
Strober, Warren - Abstract:
- Abstract: Cytotoxic CD8 + T cells mediate cellular immunity through recognition of specific antigens presented by MHC class I on all nucleated cells. Studying T cell interactions and responses provides invaluable information on infection, autoimmunity and cancer. Fluorescently labeled multimers of MHC I can be used to quantify, characterize, and isolate specific CD8 + T cells by flow cytometry. Here we describe the production and use of conditional MHC I multimers that can be loaded with peptides of choice by incubating them at a defined temperature. Multimers are folded with a template peptide that forms a stable complex at low temperature, but dissociates at a defined elevated temperature. Using this technology multiple MHC I multimers can be generated in parallel, to allow staining and isolation of large sets of antigen‐specific CD8 + T cells, especially when combined with barcoding technologies. © 2019 The Authors.
- Is Part Of:
- Current protocols in immunology. Volume 126(2019)
- Journal:
- Current protocols in immunology
- Issue:
- Volume 126(2019)
- Issue Display:
- Volume 126, Issue 2019 (2019)
- Year:
- 2019
- Volume:
- 126
- Issue:
- 2019
- Issue Sort Value:
- 2019-0126-2019-0000
- Page Start:
- n/a
- Page End:
- n/a
- Publication Date:
- 2019-07-26
- Subjects:
- CD8+ T cell staining -- MHC class I -- multimer -- peptide -- thermal exchange
Immunology -- Laboratory manuals
Medical protocols
Immunologic Techniques
Medical protocols
Immunology
Laboratory manuals
Reference works
571.96028 - Journal URLs:
- https://onlinelibrary.wiley.com/journal/1934368X ↗
http://onlinelibrary.wiley.com/ ↗ - DOI:
- 10.1002/cpim.85 ↗
- Languages:
- English
- ISSNs:
- 1934-3671
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 13588.xml