Issue Information TOC. (3rd September 2019)

• Record Type: Journal Article
• Is Part Of: Current protocols in immunology. Volume 126(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-09-03
• Subjects: Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/cpim.67
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract : Cover : In Geddes‐McAlister and Gadjeva (https://doi.org/10.1002/cpim.87 ), Overview of workflow for MS‐based proteomics profiling of neutrophils. Neutrophils are isolated and purified from bone marrow of 7‐ to 9‐week‐old male or female mice. The quality of the purification is assessed by FACS. Proteins are extracted by chemical (e.g., urea) and mechanical (e.g., sonication) cell disruption and enzymatically digested with Lys‐C and trypsin proteases. The purified peptides are measured in the first MS scan and peptide fragmentation patterns are observed in the second MS scan (MS/;MS). The data is processed and analyzed using the publicly available MaxQuant and Perseus platforms. Figure generated with Biorender.com. See e87.