High‐Throughput Analysis of Behavior Under the Control of Optogenetics in Caenorhabditis elegans. Issue 1 (2nd November 2018)

• Record Type: Journal Article
• Authors: Yu, Alex J.; McDiarmid, Troy A.; Ardiel, Evan L.; Rankin, Catharine H.
• Editors: Crawley, Jacqueline N.; Gerfen, Charles R.; Rogawski, Michael A.; Sibley, David R.; Skolnick, Phil; Wray, Susan
• Is Part Of: Current protocols in neuroscience. Issue 86:Issue 1(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-11-02
• Subjects: behavior -- Caenorhabditis elegans -- high‐throughput -- Multi‐Worm Tracker -- optogenetics; Neurosciences -- Laboratory manuals; Neurosciences; Cytological Techniques; Histological Techniques; Nervous System -- anatomy & histology; Neurosciences; Laboratory Manual; Laboratory manuals; 612.8
• DOI: 10.1002/cpns.57
• Languages: English
• ISSNs: 1934-8576

Abstract

Abstract: In this unit, we describe an inexpensive and versatile method for optogenetic stimulation of a large population of genetically engineered Caenorhabditis elegans worms while quantitatively analyzing behavior. A custom light‐emitting diode light source is used to deliver blue‐light stimuli, causing direct depolarization of neurons expressing the light‐gated cation channel Channelrhodopsin‐2, which in turn evokes behavioral responses. The behavioral responses are recorded by a high‐throughput machine vision–based tracking system, the Multi‐Worm Tracker, for detailed analysis. This approach allows researchers to bypass technical obstacles to simultaneously deliver uniform stimuli to a large number of freely behaving animals and investigate the neural underpinnings of behavior. © 2018 by John Wiley & Sons, Inc.