Chromosome Preparation in Rice (Oryza sativa). Issue 1 (3rd May 2016)

• Record Type: Journal Article
• Authors: Wang, Kai; Yu, Weichang
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 1:Issue 1(2016)
• Page Start: 67
• Page End: 77
• Publication Date: 2016-05-03
• Subjects: chromosome -- metaphase -- mitosis -- chromatin -- rice -- Oryza sativa; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20006
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Chromosomes are the carriers of genetic material in biological organisms. Each chromosome has three essential components: a centromere, telomeres, and chromosome arms. Chromosome preparation is the basic technique for studies in cytogenetics, including the analysis of chromosomal behavior, chromosomal variation, karyotyping analysis, fluorescence in situ hybridization, immunostaining, and artificial chromosome technology. In this unit, we describe the basic protocol for rice ( Oryza sativa ) metaphase chromosome preparation from rice root tips, alternative chromosome preparation protocols using cultured cells, and different chromosome spreading techniques. © 2016 by John Wiley & Sons, Inc.