Chromosome Preparation for Cytogenetic Analyses in Arabidopsis. Issue 1 (3rd May 2016)

• Record Type: Journal Article
• Authors: Mandáková, Terezie; Lysak, Martin A.
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 1:Issue 1(2016)
• Page Start: 43
• Page End: 51
• Publication Date: 2016-05-03
• Subjects: arabidopsis -- Brassicaceae -- mitotic and meiotic chromosomes -- pachytene; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20009
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Despite its small nuclear genome and minute chromosomes, the model plant Arabidopsis thaliana has become a well established model in the field of plant cytogenetics. Since 2000, most cytogenetic and epigenetic approaches have been developed for this species and its congeners. In this chapter, we describe two step‐by‐step protocols for preparing chromosome preparations from root tip meristems and generative organ tissues of A. thaliana . Although both protocols have been optimized for A. thaliana, the detailed procedures are equally applicable to other members of the genus Arabidopsis as well as other crucifer species (Brassicaceae). © 2016 by John Wiley & Sons, Inc.