Virus‐Induced Gene Silencing and Transient Gene Expression in Soybean (Glycine max) Using Bean Pod Mottle Virus Infectious Clones. Issue 2 (10th June 2016)

• Record Type: Journal Article
• Authors: Whitham, Steven A.; Lincoln, Lori M.; Chowda‐Reddy, R. V.; Dittman, Jaime D.; O'Rourke, Jamie A.; Graham, Michelle A.
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 1:Issue 2(2016)
• Page Start: 263
• Page End: 283
• Publication Date: 2016-06-10
• Subjects: gene expression -- RNA silencing -- soybean -- viral vector -- virus‐induced gene silencing; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20012
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Virus‐induced gene silencing (VIGS) is a powerful and rapid approach for determining the functions of plant genes. The basis of VIGS is that a viral genome is engineered so that it can carry fragments of plant genes, typically in the 200 to 300 base pair size range. The recombinant viruses are used to infect experimental plants, and wherever the virus invades, the target gene or genes will be silenced. VIGS is thus transient, and in the span of a few weeks, it is possible to design VIGS constructs and then generate loss‐of‐function phenotypes through RNA silencing of the target genes. In soybean (Glycine max), the Bean pod mottle virus (BPMV) has been engineered to be valuable tool for silencing genes with diverse functions and also for over‐expression of foreign genes. This protocol describes a method for designing BPMV constructs and using them to silence or transiently express genes in soybean. © 2016 by John Wiley & Sons, Inc.