Rice (Oryza sativa) Protoplast Isolation and Its Application for Transient Expression Analysis. Issue 2 (10th June 2016)

• Record Type: Journal Article
• Authors: He, Feng; Chen, Songbiao; Ning, Yuese; Wang, Guo‐Liang
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 1:Issue 2(2016)
• Page Start: 373
• Page End: 383
• Publication Date: 2016-06-10
• Subjects: rice (Oryza sativa) -- protoplast isolation -- transient expression -- BiFC and Co‐IP; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20026
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Rice ( Oryza sativa ) is not only the staple food for half of the world's population but also a model monocot plant for molecular biology studies. Although rice genes have been extensively investigated in the last two decades, the functions of many genes in the rice genome are still not known. One of the rapid and efficient approaches for determining gene function in vivo is protoplast‐based transient expression analysis. We established a rice protoplast system about 10 years ago, which has been recently used in many laboratories. This protocol is useful for protein expression, subcellular localization, bimolecular fluorescence complementation, and co‐immunoprecipitation assays. © 2016 by John Wiley & Sons, Inc.