An Improved Transient Expression System Using Arabidopsis Protoplasts. Issue 2 (10th June 2016)

• Record Type: Journal Article
• Authors: Cao, Yangrong; Li, Hong; Pham, An Q.; Stacey, Gary
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 1:Issue 2(2016)
• Page Start: 285
• Page End: 291
• Publication Date: 2016-06-10
• Subjects: Arabidopsis -- protoplast -- transient expression; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20013
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Transient gene expression in protoplasts provides a powerful tool to study protein expression, protein localization, protein‐protein association, and gene expression regulation, etc. There are several methods including electroporation, which have been reported to introduce DNA into protoplasts. However, one of the best methods used is polyethylene glycol (PEG)‐mediated transfection. Here, we describe an improved PEG‐mediated transformation method including preparation of protoplasts, PEG‐mediated transformation, and, by way of example, expression of the AtLYK5 gene (AT2G33580) in protoplasts. The protoplast transient expression system provides unique capabilities to support cell‐based experiments involved in plant biochemistry and physiology. © 2016 by John Wiley & Sons, Inc.