In Vitro Selection and Characterization of DNA Aptamers to a Small Molecule Target. Issue 4 (14th December 2017)

• Record Type: Journal Article
• Authors: Ruscito, Annamaria; McConnell, Erin M.; Koudrina, Anna; Velu, Ranganathan; Mattice, Christopher; Hunt, Vernon; McKeague, Maureen; DeRosa, Maria C.
• Is Part Of: Current protocols in chemical biology. Volume 9:Issue 4(2017)
• Page Start: 233
• Page End: 268
• Publication Date: 2017-12-14
• Subjects: aptamer -- DNA -- high throughput sequencing -- in vitro selection -- SELEX -- small molecule; Biochemistry -- Laboratory manuals; Chemical Phenomena; Biochemistry; Periodicals; Laboratory manuals; 572.078
• DOI: 10.1002/cpch.28
• Languages: English
• ISSNs: 2160-4762

Abstract

Abstract: Aptamers, synthetic oligonucleotide‐based molecular recognition probes, have found use in a wide array of biosensing technologies based on their tight and highly selective binding to a variety of molecular targets. However, the inherent challenges associated with the selection and characterization of aptamers for small molecule targets have resulted in their underrepresentation, despite the need for small molecule detection in fields such as medicine, the environment, and agriculture. This protocol describes the steps in the selection, sequencing, affinity characterization, and truncation of DNA aptamers that are specific for small molecule targets. © 2017 by John Wiley & Sons, Inc.