Using a Kinase‐Inducible Bimolecular Switch to Control Enzyme Activity in Living Cells. Issue 3 (1st October 2013)

• Record Type: Journal Article
• Authors: Mehta, Sohum; Zhang, Jin
• Editors: Mahal, Lara; Romesberg, Floyd; Shah, Kavita; Shamu, Caroline; Strano, Michael S.; Thomas, Craig
• Is Part Of: Current protocols in chemical biology. Volume 5:Issue 3(2013)
• Page Start: 227
• Page End: 237
• Publication Date: 2013-10-01
• Subjects: live‐cell imaging -- biochemical perturbation -- biosensor -- fluorescence; Biochemistry -- Laboratory manuals; Chemical Phenomena; Biochemistry; Periodicals; Laboratory manuals; 572.078
• DOI: 10.1002/9780470559277.ch130090
• Languages: English
• ISSNs: 2160-4762

Abstract

Abstract: Molecular switches have been instrumental in the development of powerful and versatile genetic tools for directly probing biochemical processes, such as intracellular signaling, within their native contexts. A molecular switch can be broadly defined as a molecular system capable of existing in either of two states (e.g., conformations), which can be converted from one state to the other by a specific input stimulus. This protocol outlines a method for using a kinase‐inducible bimolecular switch, along with live‐cell fluorescence microscopy, to directly control and monitor the activity of a specific enzyme in living cells. Curr. Protoc. Chem. Biol . 5:227‐237 © 2013 by John Wiley & Sons, Inc.